SBNeC 2010
Resumo:F.061


Prêmio
F.061EFFECT OF MATERNAL ETHANOL INTAKE DURING THE PREGNANCY AND LACTATION ON THE LOCOMOTOR AND EXPLORATORY ACTIVITIES OF THE PUPS IN ADULTHOOD.
Autores:Gabriela Momente Miquelin (FAMECA - Faculdade de Medicina de Catanduva- FIPA) ; Mariana Stuchi Graça (FAMECA - Faculdade de Medicina de Catanduva- FIPA) ; Vitória Cristina Bortolani (FAMECA - Faculdade de Medicina de Catanduva- FIPA) ; Renata Maria Álvares (FAMECA - Faculdade de Medicina de Catanduva- FIPA) ; Melina Mizusaki Iyomasa (FAMECA - Faculdade de Medicina de Catanduva- FIPA) ; Maria Luiza Rosa (FAMECA - Faculdade de Medicina de Catanduva- FIPAINEC - Universidade de São Paulo)

Resumo

EFFECT OF MATERNAL ETHANOL INTAKE DURING THE PREGNANCY AND LACTATION ON THE LOCOMOTOR AND EXPLORATORY ACTIVITIES OF THE PUPS IN ADULTHOOD. Miquelin GM1, Álvares RM1, Bortolani V1, Graça MS1, Iyomasa MM1, Rosa MLNM1,2. 1Laboratory of Neuroscience, School of Medicine of Catanduva-FIPA; 2Institute of Neuroscience and Behavior-USP, Ribeirao Preto, Sao Paulo, Brazil. E-mail-mlrosa.fameca@gmail.com OBJECTIVE: Ethanol exposure during fetal development can result in behavioral and neurological deficits. This work aimed at investigating whether ethanol consumption during the pregnancy and lactation might induces alterations on the locomotor and exploratory activities of the pups after reaching adulthood. METHODS: Female Wistar rats (150g) were exposed to 5%-10% of ethanol, increasing 5% per week (habituation), and 10% maintained for 60 days (chronic ingestion), corresponding to the period before mating (8 days), mating (10 days), pregnancy (21 days) and lactation (21 days). The control group was exposed to water. After weaning the pups from control (n=8) and alcoholic mothers (n=16) were left undisturbed in their cages (4/cage) until PND60 (500-600g) with food and water ad libitum. Then, the animals were tested in a circular open arena. The animals were put into the middle of the arena and the behavioral responses scored every minute for 5 minutes: Number of crossings (horizontal exploration) and number of rearings (vertical exploration). Control and alcoholic groups were compared by Student t-tests and the level of significance was set at p<0.05. RESULTS: The pups from alcoholic mothers showed locomotor hyperactivity during the 5 minutes in the arena with an increase of 51% of the numbers of crossings compared to control pups (p=0.002). The increased number of crossings was specifically significant on the first 3 minutes in the arena (1° min: 34%, p<0.001; 2° min: 67%, p=0.03; 3° min: 89%, p=0.001). On the vertical exploration the pups from alcoholic mothers also showed an increase of the total number of rearings during the 5 minutes in the arena when compared to control pups (31%, p=0.06). However, this increase was significant only at the minute 3 (97%, p<0.01). CONCLUSION: Maternal alcohol intake during the pregnancy and lactation induces long term changes on the pup brains leading to behavioral alterations such as locomotor hyperactivity in adulthood. Committee on Animal Research and Ethics(CEUA-School of Medicine of Catanduva): 02/08 Financial Support: Padre Albino Foundation.


Palavras-chave:  arena, ethanol, exploratory activity, FAS, locomotor hyperactivity