SBNeC 2010
Resumo:F.130


Poster (Painel)
F.130EFFECTS OF CHRONIC ADMINISTRATION OF MODAFINIL ON MEMORY CONSOLIDATION IN MICE
Autores:Helaine Arrais Fernandes (UNIFESP - Universidade Federal de São Paulo) ; Luciano Fernandes Santos (UNIFESP - Universidade Federal de São Paulo) ; Lia Rita Azeredo Bittencourt (UNIFESP - Universidade Federal de São Paulo) ; Sérgio Tufik (UNIFESP - Universidade Federal de São Paulo) ; Roberto Frussa Filho (UNIFESP - Universidade Federal de São Paulo)

Resumo

Objective: Modafinil (MOD) is a non-amphetamine stimulant that acts as a wake-promoting drug and has been approved for the treatment of excessive daytime sleepiness in narcolepsy, obstructive sleep apnea and shift workers syndrome. There is preliminary clinical evidence of a possible improving effect of modafinil on cognitive functions, such as learning and memory. In addition, the effects of this drug on anxiety levels remain unknown. Thus, the objective of the present study was to characterize the effects of post-training chronic administration of different doses of MOD (32; 64 and 128 mg/kg, i.p.) on memory of mice submitted to the plus-maze discriminative avoidance task (PM-DAT). In this animal model, mice are exposed to a modified plus-maze in which, in the training session, they receive aversive stimuli (light and cold air blow) when entering the aversive enclosed arm (Av)-but not when entering the non-aversive enclosed arm (Nav). Simultaneously, they avoid the two open arms (OA) of the apparatus. In the test session, they must remember that the Av had been aversive and avoid it. Thus, learning and memory are evaluated by the percent time spent in the Av versus Nav in the training and in the test sessions, respectively. Anxiety is evaluated by the percent time spent in the open arms (%TOA) and motor activity is evaluated by the total number of entries in all of the arms of the apparatus (NE). Methods: Swiss male mice, 3-month-old, were trained in the PM-DAT and, immediately after, received repeated administration (10 days every day) of saline (SAL) or modafinil in the doses of 32 (MOD32), 64 (MOD64) or (MOD128) mg/kg. Twenty for hours after the last saline or modafinil injection, animals were submitted to the test session. Results: The results showed that the post-training chronic administration of 32 mg/kg of MOD promoted amnestic effects since in the test session they presented an increased in the percent of time mice spent in the enclosed arm that had been aversive in the training session (SAL: 2.42±;1.43 MOD32: 29.78±9.85; MOD64: 11.16±4.19 and MOD128: 13.56±5.26). In addition, no effects were found concerning the % TOA and NE. Conclusion: Our results indicate that the chronic post-training administration of modafinil may impair consolidation of a discriminative task in mice. This amnestic effect is not associated with alterations in anxiety-like behavior or motor function.


Palavras-chave:  Memory Consolidation, Modafinil, Mice